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We assessed the potential use of Whatman FTA paper as a device for archiving and long-term storage of bacterial cell suspensions of over bacterial strains representing 61 genera, the molecular applications of immobilised DNA on FTA paper, and tested its microbial inactivation properties.
The FTA paper extracted bacterial DNA is of sufficiently high quality to successfully carryout the molecular detection of several key genes including 16S rRNA, esp Enterococcus surface proteinBft Bacteroides fragilis enterotoxin and por porin protein by PCR and for DNA fingerprinting by random amplified polymorphic DNA-PCR RAPD-PCR, dna storage long term paper fta. To test the long-term stability of the FTA immobilised DNA, of the archived bacterial samples were randomly selected following 3 years of storage at ambient temperature and PCR amplification was used dna storage long term paper fta monitor its success.
All of the samples were successfully amplified using the 16S rDNA gene as a target and confirmed by DNA sequencing. Furthermore, the DNA was eluted into solution from the FTA cards using a new alkaline elution procedure for evaluation by real-time PCR-based assays.
The viability of cells retained on the FTA cards varied among broad groups of bacteria. For the more fragile gram-negative species, no viable cells were retained even at high cell densities of between 10 7 and 10 8 colony forming units cfu ml -1and for the most robust species such as spore-formers and acid-fast bacteria, complete inactivation was achieved at cell densities ranging between 10 1 and 10 4 cfu ml The inactivation of bacterial cells on FTA cards suggest that this is a safe medium for the storage and transport of bacterial nucleic acids.
Abstract We assessed the potential use of Whatman FTA paper as a device for archiving and long-term storage of bacterial cell suspensions of over bacterial strains representing 61 genera, dna storage long term paper fta, the molecular applications of immobilised DNA on FTA paper, and tested its microbial inactivation properties.
Publication types Research Support, Non-U. Substances Bacterial Proteins Bacterial Toxins DNA, Bacterial DNA, Ribosomal Membrane Proteins Porins enterococcal surface protein, esp Bacteroides fragilis toxin Metalloendopeptidases.
DNA: The Worlds Most Efficient Data Storage Device?
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